Characterization of Diaporthe ampelina isolates and their Sensitivity to Hot-Water Treatments and Fungicides in in vitro

Diaporthe ampelina (=Phomopsis viticola) is one of the most important pathogens causing both cane/leaf spot and wood canker diseases in grape growing countries world. In this research, morphological, molecular pathogenic characterization 23 D. isolates were studied their sensitivity tested against hot-water treatments some fungicides used vineyards. Morphologically, grouped according to “W type” “G colony appearance microscopic features. characterization, beta-tubulin, calmodulin translation elongation factor (tef1-α) gene regions amplified with PCR. The nucleotide sequences analyzed using NCBI-BLAST search recorded GenBank, species identity was confirmed. Mycelial viability (46 – 50°C for 30 45 min) centrifuge tubes thermal inactivation point determined. It also (azoxystrobin, boscalid, cyprodinil, tebuconazole, azoxystrobin + cyproconazole cyprodinil fludioxonil, tebuconazole fludioxonil) vitro EC50 values calculated. morphological study results showed that all they on tissues vines. Thermal ensured at 48°C-30 min treatments. Although treatment reduced growth isolates, it did not inhibit completely 48°C-45 needed reach full eradication. Considering fungicide sensitivity, fludioxonil or containing effective suppressing mycelial fungus. However, azoxystrobin, could perform a strong inhibition when compared tebuconazole.